Quantitative and qualitative study of phenolic compounds and evaluation of antioxidant effect of Echium italicum L. leaf and flower extracts

Document Type : Research Paper

Authors

1 M.A in Organic Chemistry, Payame Noor University, Iran.

2 Associate Professor, Department of chemistry, Payame Noor University, Iran.

3 Professor, Department of chemistry, Payame Noor University, Iran.

4 PhD student of Organic Chemistry, Mohaghegh Ardabili University, Ardabil. Iran.

Abstract

The plant Echium italicum L. (Family: Boriginaceae, common name: Gaw_zaban) is one of four existing species of genus Echium in Iran. Scientific studies have indicated that some plants possess strong antioxidant activities and use of them makes the user resistant to the dangers of oxidative diseases. This study was conducted to investigate the antioxidant capacity of Echium italicum L. leaf and flower extracts from a natural habitat in Iran. The plant extracts were obtained by Soxhlet apparatus using methanol as solvent. Antioxidant activities of the extracts were evaluated using DPPH and beta-carotene tests and total phenolic compounds of the extracts were estimated both quantitatively and qualitatively using folin-ciocalteu test. The leaf and flower extraction yields were 21.3% w/w and 13.60% w/w respectively. In DPPH test, IC50 values 63.11±1.45 μg/ml and 102.81±1.08 μg/ml were obtained for leaf and flower extract respectively, showing potencies one-thred and one-fifth of that of BHT (IC50 = 19.72± 0.80 μg/ml). In beta-carotene-linolenic acid test, inhibition percentages of%57.22 and%48.74 were recorded for leaf and flower extracts, respectively, comparable to that of BHT (%89.43). Total phenolic compounds contents of the extracts equivalent to galic acid were evaluated 68.82 µg/mg and 30.25 µg/mg for leaf and flower extracts, respectively. Totally, the results of this investigation was confirmed good natural antioxidant potentials for the plant extracts

Keywords

References

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History

  • Receive Date: 13 November 2020
  • Revise Date: 20 February 2022
  • Accept Date: 03 March 2022

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